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By Mohamed Al-Rubeai, M. Al-Rubeai, F. Autuori, E M. Bruckheimer, S.H. Cho, T.G. Cotter, Z. Darzynkiewicz, M.G. Farrace, N.L. Harvey, J. Herrmann, S. Kumar, T.J. McdDonnell, A.J. McGowan, S.L. McKenna, R. O'Connor, S. Oliverio, M. Piacentini, M. Piredda, M.

ISBN-10: 354064153X

ISBN-13: 9783540641537

The purpose of this quantity is to supply an in-depth review of the cutting-edge study on apoptosis with contributions from key teams operating within the box. this kind of programme cellphone demise has got huge and quick consciousness and now's regarded as one of many most popular parts of technology. the amount covers numerous elements of the apoptotic demise method from the morphological and biochemical good points, mechanisms and genetic law to its function in pathological procedure and capability implications for biomedical examine and biopharmaceutical construction.

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It may be possible to promote cell viability by improving culture media with specific survival factors, or anti-oxidant molecules. Alternatively, the generation of more robust cell lines by genetic manipulation may prove to be a more cost effective long term option. It has already been established that the expression of Bcl-2 in a variety of non-commercial cell lines can protect them from a wide range of cytotoxic insults. In addition, a recent study has demonstrated that the stable expression of Bcl-2 in a commercially important hybridoma cell line produced a more adaptable cell line and significantly increased productivity ([180] and Chap.

An interesting feature of both HIV induced apoptosis, and apoptosis in neurodegenerative disease is the increase in oxidative stress during these conditions [176]. An imbalance in cellular redox status has also been reported to promote tumour development [177]. It is anticipated that a better understanding of both the genetic and biochemical mediators in apoptosis pathways should facilitate the development of new therapeutic options for the treatment of the various diseases in which de-regulated apoptosis plays a significant role (see also Chap.

1 Erroneous Quantitation of Apoptosis Based on the Extent of DNA Degradation . . . . . . . . . . . . . . Erroneous Classification of Apoptotic Bodies or Chromatin Fragments as Apoptotic Cells . . . . . . . . Erroneous Assumption that the Percentage of Apoptotic Cells Represents the Cell Death Rate . . . . . . . . . . . Erroneous Assumption that Apoptotic Cells Exhibit All Classical Features of Apoptosis . . . . . . . . . Problems with Distinction of Late Apoptotic vs Necrotic Cells .

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Apoptosis by Mohamed Al-Rubeai, M. Al-Rubeai, F. Autuori, E M. Bruckheimer, S.H. Cho, T.G. Cotter, Z. Darzynkiewicz, M.G. Farrace, N.L. Harvey, J. Herrmann, S. Kumar, T.J. McdDonnell, A.J. McGowan, S.L. McKenna, R. O'Connor, S. Oliverio, M. Piacentini, M. Piredda, M.

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