By Robert B. McComb
There will be without doubt that alkaline phosphatase is among the such a lot generally in vestigated of all enzymes. This has resulted from the ubiquity of its distribution, and from the convenience and sensitivity with which its job could be measured. regrettably, those wide-ranging yet usually superficial experimental stories were up by way of extensive and systematic investigations in just a couple of constrained components of the biochemistry and chemical pathology of alkaline phosphatase. the outcome has been the buildup of a systematic literature of intimidating proportions, and the inevitable rediscovery of already identified proof in regards to the enzyme. Scientists are taught early of their careers that, within the phrases of Sir John Herschel, "Hasty generalization is the bane of technological know-how. " however, moments arrive in all spheres of clinical job while generalization turns into crucial, to codify and to choose between the mass of information already amassed, and to supply beginning issues for brand spanking new advancements and new strains of research. this is often very true in a box equivalent to alkaline phosphatase examine, within which very genuine hazards exist that the seeds of primary realizing can be misplaced amidst an unexamined harvest of empirical observations. The historical past of the examine of alkaline phosphatase offers numerous cases while precious generalizations have emerged. sometimes, the conclusions drawn at the foundation of obtainable facts have been incorrect; extra often, they've got stood the attempt of extra experimentation, and constantly, they've got supplied new insights into the character and correct ties of this enzyme.
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Additional info for Alkaline Phosphatase
13,1047,1959. 22 CHAPTER 2 149. Schwartz, J. , The phosphorylation of alkaline phosphatase, Proc. Natl. Acad. Sci. , 49, 871,1963. 150. , and Wilson, I. , Evidence for a phosphoryl-enzyme intermediate in alkaline phosphatase catalyzed reactions, Biochemistry, 8, 1042, 1969. 151. Aldridge, W. , Barman, T. , The rate of formation and decomposition of phosphorylphosphatase (Escherichia coli), Biochem. , 92, 23C, 1964. 152. Fernley, H. , and Walker, P. , Studies on alkaline phosphatase: Transient-state and steadystate kinetics of Escherichia coli alkaline phosphatase, Biochem.
234 Support for this view was added by the demonstration of an inverse relationship between phosphatase synthesis by some types of E. coli and the phosphate content of the growth medium. 162 , 163 Other evidence for a transport function for alkaline phosphatase comes from the finding that some mutant strains, which lack alkaline phosphatases, are unable to grow in phosphate-poor media. 23s In addition, it has now been shown that a number of alkaline phosphatases bind orthophosphate strongly under suitable conditions.
Minor enzyme was enhanced approximately 40% when the assay was carried out in the presence of ethylenediamine tetraacetic acid (EDTA). 3. Algae In contrast to higher plants, algae contain alkaline phosphatase. 25 ,26,28 This has been demonstrated in green algae, blue-green algae, and diatoms,z6,29,3o Electron microscopy was employed 20 years ag0 25 to demonstrate alkaline phosphatase near the outer surface of the cell wall of Chlorella vulgaris. In some species of algae, a 10- to 25fold increase in alkaline phosphatase activity was achieved by growing these plants in a phosphate-poor medium;26,27,31 in others, this maneuver resulted in increased acid phosphatase.
Alkaline Phosphatase by Robert B. McComb